Gram Stainin 1. What is the crucial step that differentiates Gram negative from

Question

Gram Stainin 1. What is the crucial step that differentiates Gram negative from Gram positive bacteria during Gram staining? Why? 2. What do primary and secondary stain have as common characteristic(s)? 3. Which stain should have a darker color during Gram staining? What happens if the opposite was used? 4. A student was performing Gram staining using the procedure described in the figure below. She was trying to focus the slide after every step to know the importance of each step. Complete the figure (A to J) by indicating the color of both Gram negative and Gram positive bacteria after each staining step GRAM-NEGATIVE Fixation A. B. Crystal Violet C. D. lodine Treatment G. H. Counter stain with Safranin

Explanation / Answer

in grams staining the crucial step is Destaining as it differentiates the gram positive and gram negative bacteria .after this process only ,the bacteria which retain the colour of primary stain( crystal violet ) are gram positive and those which retain from secondary stain (safarinin) are gram negative bacteria. common characteristic of primary and secondary stain is in both the cases when the stain is added (primary and secondary) to the sample then they are incubated for one minute and are tinder with a gentle stream of water for 5 seconds.hence this is the common characteristic of primary and secondary stains. Gram positive stain should have the darker colour during gram staining as it has a cell wall containing peptidoglycan which results in purple colour stain by crystal violet.If the opposite dye (safranin)was used then it would stain counter pink. A .colourless B.colourless C.dark blue D.dark blue E.purple F.purple G.purple H.colourless I.purple J.pink.

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