1.How cell attachment and growth in PBS, media WITH the serum will result after
ID: 149770 • Letter: 1
Question
1.How cell attachment and growth in PBS, media WITH the serum will result after 48hrs and 7 days, under proper incubation of course? 2.How cell attachment and growth in PBS, media WITHOUT the serum will result after 48hrs and 7 days, under proper incubation of course?1.How cell attachment and growth in PBS, media WITH the serum will result after 48hrs and 7 days, under proper incubation of course? 2.How cell attachment and growth in PBS, media WITHOUT the serum will result after 48hrs and 7 days, under proper incubation of course?
2.How cell attachment and growth in PBS, media WITHOUT the serum will result after 48hrs and 7 days, under proper incubation of course?
Explanation / Answer
Serum containing media - Serum is vitally important as a source of growth and adhesion factors.
Serum contains many ECM proteins, which could potentially coat the wells of the plate.
At 48 hrs it attach to plate and growth are normal
At 7 days still attach to plate but medium composition started to decrease, incresae in confluency
so death of cells will occurs, the debris and death cells floating on the media.
Serum free media - Serum is not present we added appropriate nutritional and hormonal formulations.
After 48hrs it not attach to the plate and growth are normal.
After 7 days most of cells are not attach to plate and they are floating in the medium and also of death
cells and debris are formed due to shortage of nutritional composition.
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