1. A student should wash their hands for 10 seconds when practicing aseptic tech

Question

1. A student should wash their hands for 10 seconds when practicing aseptic technique.

True/False

2. When transferring culture to agar plates, it is OK to have the plate uncovered for as long as you want because this will not interfere with your results.

True/False

3. The test tube containing culture in a broth should be flamed when opening and right before closing to ensure the death of any bacteria that could contaminate the culture.

True/False

4. The CDC bio-safety 2 deals with organisms thought to be harmless as far as disease is concerned.

True/False

5. In the lab, it is Ok to have a drink out just as long as you do not open it.

True/False

Explanation / Answer

1. True.

note: aseptic technique technique like isolation of pure culture and transferring cultures, media inoculation. such practice helps to prevent contamination of experiment in laboratory.

2. False.

Note: Agar plates contains nutrients that support microbial growth and hence should be closed and sealed with paraffin immediatiely after transferring. It prevents the growth of other unwanted microbes on the agar plates.

3. True.

Note: passing the test tube through flame creates a convection current which forces air out of the tube and hence ensure prevention of air borne contaminants entry in test tube media.

4. False.

Note: Biosafety Level 2 precautions are appropriate for facilities that work with a broad range of indigenous moderate-risk agents known to cause human disease (e.g., Hepatitis B virus, salmonellae, and Toxoplasma spp.)

5. false.

Note: There should be no drinks, food stored in the lab to prevent unwanted contamination.

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