With reference to the metabolic pathways involved, explain your observations of

ID: 147981 • Letter: W

Question

With reference to the metabolic pathways involved, explain your observations of GLUCOSE CONCENTRATION across the strains under the different conditions:

Concentration of Extracellular Glucose in growth medium Growth time of cultures Cell concentration cells/mL rowth (Tm 3.75 mM No. Carbon O2 Strain of EtOH Presence at cessation of Initial (To)Final (Tm) source 30 1Glucose 8.450 0.022 0 20 Yes 0.35 8.399 0.017 20 2 Glucose Yes 0.55 8.637 0.0189 20 Yes 0.5 8.688 0.0183 30 Pyruvate 5.2 NIA NIA 0 NIA NIA NIA 0

Explanation / Answer

When metabolic pathways are involved , here only two conversions are possible

Either the glucose will get converted to Carbon dioxide and water or it will undergo alcoholic fermentation anf form ethanol.

Discussing the case one by one-

1.Glucose -present , Oxygen -present

a)In strain Gr, when no ethanol is present the final concentration of extracellular glucose is very less as compared to initial concentration.This is because glucose + water gets converted to carbon dioxide and water.Therfore, all of the glucose gets used up.

-b)In strain Pe, when ethanol is present, then glucose undergoes alcoholic fermentation and forms more ethanol and gets used up. Thats why the final concentration here is also very less as compared to intial concentration of extracellular glucose.

2.Glucose - present , oxygen -absent

In case of both strains Gr and Pe, ethanol is present , so it will undergo alcoholic fermentation as same as case 1b. As a result , here also the final concentration is less than initial one. The concentration differs by marginal numbers.

3. Pyruvate- presen, oxygen -present , Ethanol -absent

In this case as pyruvate is present as carbon source and not glucose, the cell will undergo aerobic respiration,so therefore, the extracellular glucose cannot be determined and therefore the values are not available for both the strains.

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With reference to the metabolic pathways involved, explain your observations of

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