2. To determine the viability percentage of an MDA-MB-231 cell culture, a resear
ID: 147898 • Letter: 2
Question
2. To determine the viability percentage of an MDA-MB-231 cell culture, a researcher removed 20 ul of trypsinized cells and added 20 ul of Tryptan Blue to it. The viable and non-viable cells were counts gave rise to the following data (50 points) Hemocytometer Viable Cells Square Non-viable cells 4 15 18 20 12 4 A) Calculate the viability percentage of the MDA-MB-231 cell culture B) Calculate the average number of viable cells/ml in the MDA-MB-231 culture C) Calculate the average number of non-viable cells/ml in the MDA-MB-231 culture D) What is the concentration of viable cells in the Hola culture with the standard deviation? Based on your viability calculations, do you think this cell culture is healthy and growing exponentially? E)Explanation / Answer
A) dilution factor = (Volume of trypisinized cell + Volume of trypan blue ) / Volume of trypsinized cell = ( 20+20)/20 = 40/40=2
live cell count in all square = 11+15+18+20/4 = 16
cells / ml = 16 X 104 X 2
= 32 X 104
now dead cell count is 4+9+12+8/ 4= 8.25
cells / ml=8.25 X104 X 2= 16.25 X104
thus total cell count is = 32 X 104 +16.25 X104 =48.25 X 104
now the viability percentage is 32 X 104/ 48.25 X 104 X 100=66.32%
B)avarage no of viable cell/ml = average of viable cell count per square x Dilution Factor x 104
= 11+15+18+20/4 X 104 X 2= 32 X 104
c) avarage of non viable cells/ml =4+9+12+8/ 4 X104 X 2= 16.25 X104
d) Cell count per milliliter ÷ dilution factor = Cell concentration
viable cell concentration = viable cells count /ml divided by dilution factor
= 32 X 104/ 2 = 16 X 104
SD = 3.9
E)for a cell to be healthy the viablitiy generally stays between 85 to 90%. But in this cell line the determined viability is 66%, thus the cells are not in good condition not growing exponentially.
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