4. Which of the following are true statements regarding cDNA and genomic librari

Question

4. Which of the following are true statements regarding cDNA and genomic libraries? Please answer and EXPLAIN.

a) Genomic libraries prepared from heart tissue and liver cells from the same individual will be identical.

b) Genomic libraries prepared from liver cells from two different individuals will be identical.

c) cDNA libraries prepared heart tissue and liver cells from the same individual will be identical.

d) All sequences present in a genomic library prepared from heart tissue from one individual will be present in a cDNA library from heart tissue of that same individual.

Explanation / Answer

A cDNA library is a combination of cloned cDNA fragments inserted into a collection of host cells. This cloned DNA fragments together constitute some portion of the transcriptome of the organism and are stored as a ' library '.  

cDNA s created from a mature mRNA from a eukaryotic cell with the use of reverse transcriptase.

cDNA library lacks the non-coding section and regulatory elements found in genomic DNA. Genomic DNA libraries provide more detailed information about a particular organism but are more resource-intensive to generate and maintain.

A genomic library is a collection of the total genomic DNA from a single organism.

Hence, the true statement will be [a] Genomic libraries prepared from the heart tissue and liver cells of the same individual will be identical , and [c] cDNA libraries prepared from heart tissue and liver cells from the same individual will be identical.

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