Esp3 I 2 paB I 179 a Eco0109 1 2671 Aat II 2617 Nde I 183 Nar I/Kas I 235 Ssp I

Question

Esp3 I 2 paB I 179 a Eco0109 1 2671 Aat II 2617 Nde I 183 Nar I/Kas I 235 Ssp I 25oi 1 245 Fsp 1 256 14 1 276 Puu 11 306 ..BerF | 387 Eeo57 1 2381 Polylinker cloning sites 396-454 Xmn 1 2294 Beg I 2215 Sca I 2177 Puu I 2066 Ava II 2059 pUC19 2,686 bp Puu 11 628 1641 Sap I 683 BsrD I 1935 Fsp I 1919 A1 781 AL III 806 Ava II 1837 BgI 1813 Bpm 1 17 Bs 1 1779 Drd I 908 Bsa I 1766 Bsrb I 1753 P11108 I 1715 Eam1105 I 1694 Mme1 1180 HaiEII 1387 Eco57 1 1333 400 Ban In Ava I Xma 1 420 440 480 Ect136 II Sac I Sse8387I Pst Sma I Xba I Hind III agtgaatCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGegtaatcatggtcat Kpn I AccB5 I BamHI Sal I Hine 11 Acc I Sph I EcoR I Apo I BspM I 1. How many band(s) would you get on a gel if you cut pUC19 with Pstl'?

Explanation / Answer

1. If pUC19 is cut with PstI then we would get only one band. As there is no insert only the plasmid vector present, the result of getting one band is correct. PstI will cut at its particular recognition sequence and leave the plasmid with cut open. Hence one band will be got as no other fragments cut out.

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