a) Explain in your own words why the binding curve illustrated in figure 4.12 an
ID: 147085 • Letter: A
Question
a) Explain in your own words why the binding curve illustrated in figure 4.12 and others show a sigmoidal curve rather than a linear curve, and how this relates to repressor functionality
b) Describe an experiment that would allow you to accurately determine the concentrations of bound operator DNA and free operator
TWO 79 100% DNA bound sigmoid curve 0% repressor concentration Figure 4.12. Binding of repressor to DNA. The dashed line is an example of an ordinary so-called Michaelis-Menten curve. It would describe the binding reaction if the repressor dimer were sta- ble at all concentrations, and if a single dimer were to bind the operator site O,1. The sigmoid ually observed for the à binding reaction. [Strictly speaking, the "repressor con- ion" should be the concentration of free repressor (R), that is, the total repressor (R,) minus o operator (Op). But in this experiment the repressor is in large excess over operator, curve is that actually observed for the and so (R) ~ (R,).JExplanation / Answer
a.The graph is sigmoid curve because rate reaction is dependent upon the repressor concentration. As, the concentration of repressor increase, first the rate of binding to operator increase, but after some time, the reaction rate become saturate.
This relate to the receptor action because whether the repressor or RNA polymease will bind to the operator depends on the concentration of repressor and RNA polymerase.
b. Fluroscence emissions factor is the experiment that is used to see the changes involved when repressor binds to operator and without binding to the repressor in the presence and absence of inducer. The shift in spectra of fluroscence will show the concentration of bound operator DNA and free operator.
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