11. NADP is an essential cofactor for many cellular processes. Because it is not

Question

11. NADP is an essential cofactor for many cellular processes. Because it is not transported, exogenous NADP cannot supplement mutants unable to synthesize intracellular NADP. Five independent mutations were obtained that affect the synthesis of NADP. The properties of the mutations are described in the table below (where + indicates growth on rich medium, - indicates that no growth on rich medium, and-/+ indicates weak growth on rich medium) Growth temperature 42°C Mutation 30°C 30 42°C 42 30oC nad nad-2 nad-4 nad-7 nad-1 nad-2 nad-1 nad-4 nad-1 nad-7 01

Explanation / Answer

nad-I shows growth on rich medium at a temperature of 30 degrees and no growth on 42 degress. So it indicates the mutant is temperature sensitive.

nad-2 shows the same characteristics as nad-I, growth on 30 degress and no growth on 42 degress. Therefore, this mutant is also temperature sensitive.

nad-4 on the other hand shows growth on 42 degress and no growth on 30 degress. This indicates the mutant is cold sensitive.

nad-7 shows the same characteristics as nad -4, which means it is also cold-sensitive.

All of the above mutations are either due to deletion or insertion because , they are a type of conditional mutation in which the mutant allele causes the mutantion in only a certain condition and temperature sensitiveness is a type of conditional mutation in which one mutant can grow on one temperature and die on another.

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