C. Lipids Table 3.3- Please indicate the color of your result & whether it was +
ID: 146961 • Letter: C
Question
C. Lipids Table 3.3- Please indicate the color of your result & whether it was + or - for the Sudan IlI est. Code Letter Sudan IIl's Result Sample Distilled Water Margarine Crisco Whipped Cream Corn Starch Unknown # U1 10. Why does the Sudan IlIl reagent bind to the samples that they did? Which compound(s) yielded the most positive result and does this support what you know about the lipid or fat content of these substances? 11. 12. Why is it important to include distilled water as a test sample in each of the tests (Benedict's, Lugol's, Biuret and SudanExplanation / Answer
10.sudan 3 is a dye which has similar structure with azobenzene,the tautomeric difference marks the significance in its mode of action,makes insoluble in water,alkali or dilute acid.slightly soluble in alcohol,readily soluble in hot glacial acetic acid.sudan dye responds to non polar and turn non polar lipid to red.
11.From the above given samples,whipped cream gives the most possible result.This is because sudan dye responds for oil or lipids or lipoprotein or lipid particles in the aqueous layer that will dissolve sudan dye.With this dye,immediate oxidation is unclear and also posses low melting point for oils.
12.Distilled water decolourises and seperates aqueous layer from non aqueous layer by forming a junction.whereas in case of sudan dye for lipophilic molecules where unreacted oil drops float on the top and emulsion of oil and water acts as emulsifying agent.i.e 95% ethanol.
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