O 3 O 4 QUESTION 15 Consider the plasmid represented below. Which statement woul
ID: 146826 • Letter: O
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O 3 O 4 QUESTION 15 Consider the plasmid represented below. Which statement would be TRUE for a gel run on the fragments created by the enzymes? g10.jpg O Lane B would have more bands than Lane A O Lane A would have more bands than Lane A+iB O The band representing the longest fragment would be found in Lane A+8 The band representing the longest fragment would be found in Lane A+B QUESTION 16 You are studying the trafficking of a protein in mammalian cells and want to find out whether the protein migrates from the cytoplasm to nucleus in response to treatment. Avallable to you is a cDNA clone of the protein of interest, cloning vectors and a confocal/fiuorescence e. Place the following steps in the order in which you would proceed in attempting to solve the problem cells with a confocal/fluorescence microscope and compare treated cells to untreated cellsExplanation / Answer
Lane B will have 2 bands where as lane A will have three bands. So, option A is wrong.
Lane A will have 3 bands where as lane A+B will have 5 bands. So, OPTION B is wrong
Option C and D are written as same and both are wrong. Longest fragment would be found in lane A that is of 5000bp.
Lomgest fragment found in lane A+B would be of 4200 bp.
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