Storage Store all components at-20 °C Unit Definition: One unit incorporates 10

Question

Storage Store all components at-20 °C Unit Definition: One unit incorporates 10 nmol of total deoxyribonucleoside triphosphates into acid- precipitable DNA in 30 min at 74 °C Reagents Provided Taq DNA Polymerase, Catalog Number D6677 5 unitslpL in 20 mM Tris-HCI, pH 8.0, 100 mM KCI, 0.1 mM EDTA, 1 mM DTT, 0.5% TWEEN° 20, 0.5% IGEPAL® CA-630, 50% glycerol 250 unit:s . 10x PCR Buffer, Catalog Number P2192 1.5 ml 100 mM Tris-HCl, pH 8.3, 500 mM KC, 15 mM MgCl2, 0.01% (w/v) gelatin . 10x PCR Buffer without MgCl2 Catalog Number P2317 100 mM Tris-HCl, pH 8.3, 500 mM KC . Magnesium chloride solution, 25 mM Catalog Number M8787 Water, PCR Reagent Catalog Number W1754 Deoxynucleotide Mix, Catalog No. D72950.25 ml 10 mM dATP, 10 mM dCTP, 10 mM dGTP, 10 mM TTP .

Explanation / Answer

Every substance is express with some international units or concentration. Concentration may be in percentage (%), mM, mg/mL, ratio etc. In the given list of chemicals only the Taq DNA polymerase is an enzyme. An enzyme is always expressed in the enzyme unit (U/EU). An enzyme unit is a unit for the amount of a particular enzyme.
One U is defined as the amount of the enzyme that produces a certain amount of enzymatic activity, that is, the amount that catalyzes the conversion of 1 micromole of substrate per minute at a given temperature. Optimum activity of an enzyme depends on a particular temperature/a particular range of temperature. Below that temperature enzyme shows little/no activity. Similarly, at higher temperature enzyme loses its activity due to denaturation with the rise in temperature. The optimum temperature for activity of Taq DNA polymerase is 75- 80OC. With a half-life greater than 2 hours at 90-95OC. For a user, it is essential to know the optimum temperature of activity and half-life temperature of its DNA polymerase enzyme, based on this information they can make their PCR reactions.  
Taq DNA polymerase always provided in liquid. It is essential to mention 1ul contains how many units

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