Two mutations that affect plaque morphology in phages (a and b\") have been isol

Question

Two mutations that affect plaque morphology in phages (a and b") have been isolated. Phages carrying both mutations (a b") are mixed with wild-type phages (a' b') and added to a culture of bacterial cells Subsequent to infection and lysis, samples of the phage lysate are collected and cultured on bacterial cells. The following numbers of plaques are observed in the table below. Plaque N phenotype a* b a*b a b a b Number 2043 320 357 2134 Using the data above, calculate the recombination frequency between the a and b genes. Number

Explanation / Answer

Answer:

Hint: Recombinant progeny number is lesser always than the parental combinations.

So that Recombinant progeny are a+b- and a- b+.

Recombination frequency =( no. of Recombinants / Total progeny)*100

= (677/ 4854)*100

= 13.95%

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