6) Analysis of Tryptic digestion materials (10 pts) Perform a tryptic digestion

Question

6) Analysis of Tryptic digestion materials (10 pts) Perform a tryptic digestion on the sequence of C/EBP-alpha above. You may do it manually or you may use a computational tool to assist you. If you use a computational tool, be sure to provide proper attribution to the program. a) Show the digestion pattern for this protein b) Choose a peptide fragment that is approximately 8-10 amino acids in length and draw the full structure of this peptide. c) Calculate the molecular weight of this peptide by hand or by using a tool from the ExPASy web page. d) What is the net charge of this peptide at pH 2, pH 7 and pH 9

Explanation / Answer

answer for a)

reason: The tryptic digetion is the digetion of protein using trypsin. Trypsin is obtained from trypsinogen. Trypsin digetion has to be done for protein adsorption.

Trypsin digest the peptide in carboxy terminal of arginine and lysine residue.

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