A : 1 & 2 B : 4 & 5 C : 1 & 2 and 4 & 5 D : 2 & 3 and 4 & 5 E : 2 & 3 Q1: Using

Question

A: 1 & 2

B: 4 & 5

C: 1 & 2 and 4 & 5

D: 2 & 3 and 4 & 5

E: 2 & 3

Q1: Using the table shown, identify which proteins would migrate together during SDS-PAGE in the absence of 2-mercaptoethanol Molecular Weight Protein Net charge Number of Identical Disulfide at pH 7 subunits subunits bonds 50,000 No 2 50,000 (25,000 for 2 each subunit) 25,000 Yes Yes 4 100,000 (50,000 Yes between subunits Yes between all subunits No for each subunit) 5 100,000 (25,000 4 No for each subunit)

Explanation / Answer

Answer:

protein with large molucules would migrate together during sds-page in the absence of 2-marcaptoethanol.

Hence ,option(b) is correct answer.

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