SLOHCDDFRE HRPAYRGPDSYSSVIASHEPCDPEEMTRVWNECRSANYA ORIGIN 1 ctgtaaaaca attttttag
ID: 144313 • Letter: S
Question
SLOHCDDFRE HRPAYRGPDSYSSVIASHEPCDPEEMTRVWNECRSANYA ORIGIN 1 ctgtaaaaca attttttaga tttgtaagca ctcaatttga atgcttttag tcttagttat 61 tttatttttc gtacggtaga tgggcgttta gtattttaac cgcatcacgg tcacactggc 9 121 ggatttgttt ttattttggt cgcc tcat cettct ctqaact taga cttcactac gaacg atgacactgg a a gttgace agectgegeg getcacgatg ctcataaaga gegeggtggg caattegeg ccattc 45..1122 /gene-"br /locus_tag- "Dmel CG4934" /gene synonyn "brc; Brn; CG4934; DmelICG4934 EG:EG0007.6 fs (1)107; fs (1)A107; 1(1)6P6 /EC number=" 2-4·1·- · note- "CG4934 gene product fron transcript cG4934-RA; CG4934-PA; brn-PA; gene B /codon start l /product-"brainiac" /protein id- NP 476901.1 /db_xref "FLYBASE: FBpp0070606 /db_xref- "GencID: 3135 /db xref- "FLYBASE: Fagn0000221" /translation- "MOSKHRKLLLRCLLVLPLILLVDYCGLLTHLHELNFERHFHYPL NDDTGSGSASSGLDKFAYLRVPSETAEVPVDQPARLTMLIKSAVGNSRRREAIRRTWG agacattet qca ct atcagttca atc a gttctatctt ttegtggacg acgactacta tgttteggce aagaacgtge teaagtttet gggcaggggg at acccettcga tc cttccggttc aagcgggca ttteccttea gcactgcgac gactteeget tecateggce ggegtacaaa acqaat to atec cqaçcagat acgcgggtat ggaacga aattac agcaaga acaggcagga 1141 ctgaagageg ctgeagattg tacatatata cgaacgagac agcacctaga caacagcctt 1201 ataaagtata ttaggagcag tggatgatac ctcaaccgtt cagtgagact ttaaaccaag 1261 cagetggtge taatagtgea agatcgcaag agtaattcaa ttctaattgt aagegtaatg 1321 ataaagcagt tgattcaatt cgtatatata gagattatgt gcaaatgtcg gataacgtat 1381 gtgtggtctg atacctttga atgcaatata aagttgatta acataggaaa gcgaataaad 1441 aacttaaaat ASDOFNRSEFYLFVDDDYYVSAKNVLKFLGRGROSHOPELLFAGHVFOTSPLRHRFSR NYVSLEEYPFDRMPPYVTAGAFILSOKALROLYAASVHLPLFRFDDVYLGIVALKAGI SLOHCDDFRFHRPAYKGPDSYSSVIASHEEGDPEENTRVNNECRSANYAExplanation / Answer
Hi, the primers will amplify the regions highlighted in the question. That is rhe coding region beginibegeith ATG and ending with TAG. So to the forwatf primer asd 20 bp from ATG...for reverse primer the region before TAG most be reverse complemented.
Forwarf- 5' GTGTATAAGCTT ATG....till gct 3'.
Reverse- 5' ATTTAYGYTACC ctacgcgtaattggcggatcgg 3'
This region has exactly 1078 Bp.
It is calculated by counting the number bases from ATG to end of highlighted region. Use the numbers on right side of sequence for references. Each group correspondence to 10 bases and in each line has 60 bases.
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