2) Use the Biocatalysis/Biodegradation database (http://eawag-bbd.ethz.ch) to in

Question

2) Use the Biocatalysis/Biodegradation database (http://eawag-bbd.ethz.ch) to investigate the differences between the aerobic and anaerobic biodegradation of ethylbenzene (EB). For some questions, it might be easier to look at the graphic format of the pathways Evaluate all possible pathways and focus only the pathways that lead to complete biodegradation **Hint. Make sure that you continue to click on intermediate metabolites to follow the pathway to completion for those pathways that lead to complete biodegradtion. a. What reaction leads to the breakage of the ring for aerobic and anaerobic cells? b. For aerobic catabolism by Pseudomonas putida 39/D, what is the net gain (or loss) of NADH and ATP in the steps converting EB to intermediary metabolites (e.g., metabolites that can enter TCA- denoted as "Intermediary Metabolism- KEGG" on the website)? Document your accounting on a reaction-by-reaction basis. Note that you will need to click on reaction details from the graphical format of the pathway to track NADH and ATP

Explanation / Answer

a]Breakage of the ring for aerobic and anaerobic

2,3-Dihydroxyethylbenzene -----> 2-Hydroxy-6-oxoocta-2,4-dienoate

Benzoyl acetyl-CoA -----> Acetyl-CoA + Benzoyl-CoA

b]Net gain (or loss) of NADH and ATP

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H + H+

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate

ATP + pyruvate = ADP + phosphoenolpyruvate

Total gain : 4 ATP and 1 NADH

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