3. Shown below is an image of a common commercially available 1kB DNA ladder (pr

Question

3. Shown below is an image of a common commercially available 1kB DNA ladder (produced by Thermofisher). The concentration of DNA within this ladder is 0.5ug/uL. The amount of each band found within 0.5ug of the ladder is shown in the middle column (ng/0.5ug) along with the percent of the total shown in the last column. Given this information answer the following questions GeneRuler 1 kb DNA Ladder O'GeneRuler 1 kb DNA Ladder, ready-to-use bp ng/0.5 10000 30.0 6.0 8000 30.0 6.0 0.0 14. 6.0 70.0 14.0 2500 25.0 5.0 2000 25.0 5.0 1500 25.0 5.0 1000 60.0 12.0 750 25.0 5.0 500 25.0 5.0 250 25.0 5.0 0.5 uglane, 8 cm length gel 1XTAE, 7Vicm, 45 min Inspection of the 250bp band and the 2500bp band reveals that the same amount of DNA (25ng) leads to a "brighter" band at 2500bp relative to the band at 250bp Assuming this gel was stained with an "Intercalating" dye (like SYBR safe or ethidium bromide) how can you explain this data? (2.5 points) a.

Explanation / Answer

A Most of the times it will bind near the base pairs.

At the tiny size they dont all migrate at the same rate

Due to presence of ethidium bromide and electrophoresis ,darker bands are obtained.

B By comparing bands in a sample to a DNA ladder ,we can determine their approximate sizes.

The brighter bands are roughly above 700 base pairs.

C concentration-0.5ug/ul

It is stored at -20°c

It is produced using specially designed plasmid

It will bear phenotypical contraction

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