BIO 332: BIOINFORMATICS Exercise 2: An Exercise on Sequence Alignment In the cod
ID: 143945 • Letter: B
Question
BIO 332: BIOINFORMATICS Exercise 2: An Exercise on Sequence Alignment In the codes of life, mutation events are observed as substitution, insertion, deletion. The following are amino acid sequences of pancreatic ribonuclease from three different species. Establish homology manualy and show manually and show your alignment, then answer the following questions Given SPECIES-1 KESPAAMKFEROHMDSGSTSSSNP >SPECIES 2 KESEAMKFEROHMPSGSTSSSNPTYCNOMMKRFNMMTOGWCKPVNTFVHEP SPECIES 3 VNTFVVHEP KESEAMKGEROHMPSGSTSSNPTYCNOMMKRFNMTOGWCKPP SHOW YOUR ALIGNMENT BELOW: Answer the following questions: 1. What is the percent age sequence similarity between Species 1 and Species 2? 2. What is the percent sequence similarity between species 2 and Species 32 3. How many events of insertion and deletion do you observe in this alignment? 4. Which is more related to whom? Draw a hypothesis for the inferred relationship.Explanation / Answer
1) Between sequence 1 and 2, there are 23 similarities out of 51 amino acids. So, the percentage sequence similarity is,
= 23/51
= 0.451
= 45.1%
2) Between sequence 2 and 3, there are 23 similar sequences. So, the percentage similarity here is also 45.1%.
3) There are 4 events of insertions or deletions in this sequences.
4) The percentage similarity between sequences are same. So, both 1 and 2, as well as 2 and 3 are equally related.
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