which if he following is true about detecting proteins from enzyme activity func
ID: 143922 • Letter: W
Question
which if he following is true about detecting proteins from enzyme activity function?1) mass of the protein must be known 2) uv-via absorption spectroscopy must be used 3) the activity can be monitored either by consumption of reactants or formations of product 4) all the above 5) none of the above which if he following is true about detecting proteins from enzyme activity function?1) mass of the protein must be known 2) uv-via absorption spectroscopy must be used 3) the activity can be monitored either by consumption of reactants or formations of product 4) all the above 5) none of the aboveExplanation / Answer
Answer) 4
As for detecting protein in the solution, it needs option 2) i.e., uv-via absorption spectroscopy must be used. There are many methods to detect proteins, some of them are listed below:
Bicinchoninic acid method: The purple colored complex of copper and protein is measured at maximum absorbance in 562nm.
Bradford assay: Coomassie blue dye binds to basic and aromatic amino acids in protein molecules to form a blue colored complex which is measured at maximum absorbance of 465-595 nm.
Warburg Christian Assay: It exploits the ability of aromatic amino acid to absorb UV light at 280nm. It measures the ratio of absorbance 280nm/260nm. Nucleic acids absorbs UV light at 260 nm. Thus, it the determines even the contamination of the protein solution by nucleic acids.
And for estimation of enzyme activity, option 1) and 3) are needed.
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