Texas Southern University CLSC 359- Microbial Human Disorders-Quiz-1 Clinical La

Question

Texas Southern University CLSC 359- Microbial Human Disorders-Quiz-1 Clinical Laboratory Science Name Instruction: Selective ONE best Answer: 1. The function of a cell wall is to a. regulate the transport of macromolecules in and 2. Two types of specimens can use preservatives to maintain them until they can be delivered to the laboratory. They are: out of the cell provide rigidity and strength to the exterior of the urine and stool b. urine and vaginal secretions c. stool and throat cultures. d. pus from a wound and vaginal secretions 4. When using the isolation streaking technique, the cell provide reserve energy to the eukaryotic cell. protect the eukaryote from predators C. d. 3. Microaerophiles are bacteria that grow a. in low oxygen concentrations greatest concentration of organisms (if present) wil be in which quadrant? a. Fourth b. Third c. Second d. First 6. What technique is used to isolate bacterial colonies b. in high carbon dioxide concentrations c. in reduced oxygen concentrations but increased carbon dioxide concentrations. d. where little or no oxygen is present. S. Anaerobes are bacteria that grow: when inoculating organisms onto an agar plate? a. b- c. in increased carbon dioxide concentrations r Homogenization a. b. Centrifugation c. Isolation streaking d. Culture streaking 8. Most bacterial cultures are incubated at what in low oxygen concentrations. in reduced oxygen concentrations but increased carbon dioxide concentrations. d. 7. where little or no oxygen is present. Differential media support the grouping of microbes based on temperature range? r 25" to 27" r 30" to 40°C r 35° to 37°C a. | a. | b, c. d. different characteristics demonstrated in the media. allow the growth of fastidious microbes through the addition of certain growth enhancers. support the growth of most nonfastidious 40 to 42 C microbes support the growth of one type or group of microbes, but not another All the following principles of specimen collection are10. Enriched media fundamental to ensuring appropriate specimen management except: r collect the appropriate quantity of a. support the grouping of microbes based on different characteristics demonstrated in the media. specimen.b.allow the growth of fastidious microbes through r label the specimen accurately with the specific natomic site and the patient information. the addition of certain growth enhancers. c. support the growth of most nonfastidious select the correct anatomic site to collect the microbes. d. support the growth of one type or group of ecimen. it is acceptable to delay transport of the specimen microbes, but not another. the laboratory if it is in transport media

Explanation / Answer

1 (a) The best possible answer as it regulates the transport of molecules in and out of the cell through different channels and pores.

2 (a)Urine and stool

Preservatives like boric acid hydrochloric acid and acetic acid allow urine to be kept at room temperature and preserve for 1-3 days.For stools 10% aqueous formalin can be used.

3) (a)In low oxygen concentrations

Microaerophiles show only aerobic growth and require minimum amount of oxygen for growth.

4 (a) First quadrant

Because this method thins out specimen and isolated colonies are obtained.So till fourth quadrant very thin colony will be otained

5(a) LITTLE OR NO oxygen concentrations.

Anaerobes do not require oxygen for growth.

6(c) Isolation streaking

As colony of bacteria is isolated using streaking.

7(a) Supports the grouping of microbes based on different charcaterstics demonstrated in the media.

All other options are selective media

8(c) 35-37 *C

This is the optimum temperature for growth

9(d)

10(b)

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