Tet Bor She Picture T Step 1: The following table shows a partial DNA, mRNA, tRN

Question

Tet Bor She Picture T Step 1: The following table shows a partial DNA, mRNA, tRNA and amino acid sequence for part of a gene associated with a disease. Answer the following questions to gain insight on how to complete the sequences. Fill in the table below; include the 5' and 3' ends of each DNA/RNA sequence and the C terminal and N terminal ends for the amino acid sequences. Once the table is complete and you have obtained the single letter amino acid sequence, continue to step 2. Tip: you may want to use a pencil. DNA Double Helx mRNA U G tRNA anticodon Amino Acid Single Letter AA THR MET GLU VAL CYS 1.) mRNA may be transcribed from either strand of the DNA molecule. Therefore, it is important to determine which strand is the coding strand and which strand is the template strand. From the information in the table, are you able to identify which strand is which? How? 2) What will be the only difference between the DNA coding strand sequence and the rnRNA sequence? 3.) What will be the only difference between the DNA template sequence and the tRNA anticodon sequence? 4.) Which direction will our mRNA molecule be TRANSLATED: left to right or right to left? How do you know? 138% Sec 1Pages:1 of 2 Words:96 of 381

Explanation / Answer

1)Template strand- we called the DNA strand as template strand from where the nucleotide bases are copied at the time of mRNA synthesis. Template strand runs in the direction of 3'-5'. Whereas, the opposite strand which runs in the direction 5'-3' is called coding strand. coding strand is not transcribed.

2) Both DNA coding strand and mRNA is complimentary to the template strand. But in case of mRNA there will be no thymine (T), and there will be uracil (U) present at the place of thymine.

3) The only difference between DNA template and tRNA anticodon sequence is, that thymin(T) of DNA will be replaced by Uracil (U) in tRNA anticodon sequence.

4) As in the transcription, RNA polymerase is capable of adding nucleotides only to the 3' end of the growing mRNA chain, thus mRNA synthesis occurs in 5'-3' direction.

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