PART 3A: NUCLEOTIDES AND MUTATIONS 1. Using the sequence provided below answer t

Question

PART 3A: NUCLEOTIDES AND MUTATIONS 1. Using the sequence provided below answer the following questions A. Write the amino acid sequence for the DNA strand below. Assume this is the coding strand, and the first codon starts with the first base 5' -ATGACGTGCTAT- 3' N- MET-THR-CTS-TR- Below is a series of mutations using the coding strand from above. Write out the new amino acid sequence for each mutation. B. With an insertion: ATGAACGTGCTAT C. With a nonsense mutation: ATGACGTGATAT D. With a point mutation: ATGACGTGCAAT

Explanation / Answer

1) A) AUG ACG UGC UAU

N'- MET - THR - CYS - TYR - C'

B) AUG AAC GUG CUA U

N'- MET - ASN - VAL - LEU - C'

C) AUG ACG UGA UAU

N'- MET - ASN - STOP

D) AUG ACG UGC AAU

N'- MET - THR - CYS - ASN - C'

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