Any guidance or explanation for the answer (cell biology) to this question would

Question

Any guidance or explanation for the answer (cell biology) to this question would be extremely helpful. Thank you so much!

1.) You've sequenced the genes for two newly discovered GLUT1 alleles, GLUT1A and GLUT1B. The gene encoding GLUT1A has a single missense mutation that changes GLN161 to E. The gene encoding GLUT1B has a single missense mutation that changes Thr310 to S. Otherwise, the amino acid sequences of GLUT, GLUT1A and GLUT1B are identical. Based solely on this information, what can you most likely predict about how, or if the Kms of GLUT1A and GLUT1B to bind glucose would differ from the Km for GLUT1?

A.) Both GLUT1A and GLUT1B would most likely have significantly higher Kms than GLUT1.

B.) Both GLUT1A and GLUT1B would most likely have significantly lower Kms than GLUT1.

C.) The Km for GLUT1A is most likely to be significantly higher than the Km for GLUT1. The Km for GLUT1B is most likely to be about the same as the Km for GLUT1 or perhaps only slightly higher.

D.) The Km for GLUT1B is most likely to be significantly higher than the Km for GLUT1. The Km for GLUT1A is most likely to be about the same as the Km for GLUT1 or perhaps only slightly higher.

E.) GLUT1, GLUT1A and GLUT1B would most likely all have the same Km.

Any guidance or explanation for the answer (cell biology) to this question would be extremely helpful. Thank you so much!

1.) You've sequenced the genes for two newly discovered GLUT1 alleles, GLUT1A and GLUT1B. The gene encoding GLUT1A has a single missense mutation that changes GLN161 to E. The gene encoding GLUT1B has a single missense mutation that changes Thr310 to S. Otherwise, the amino acid sequences of GLUT, GLUT1A and GLUT1B are identical. Based solely on this information, what can you most likely predict about how, or if the Kms of GLUT1A and GLUT1B to bind glucose would differ from the Km for GLUT1?

A.) Both GLUT1A and GLUT1B would most likely have significantly higher Kms than GLUT1.

B.) Both GLUT1A and GLUT1B would most likely have significantly lower Kms than GLUT1.

C.) The Km for GLUT1A is most likely to be significantly higher than the Km for GLUT1. The Km for GLUT1B is most likely to be about the same as the Km for GLUT1 or perhaps only slightly higher.

D.) The Km for GLUT1B is most likely to be significantly higher than the Km for GLUT1. The Km for GLUT1A is most likely to be about the same as the Km for GLUT1 or perhaps only slightly higher.

E.) GLUT1, GLUT1A and GLUT1B would most likely all have the same Km.

Explanation / Answer

Km(michaelis constant) is the concentration of substrate which allows the enzyme to achieve half Vmax.An enzyme with high Km has low affinity for its substrate and requires a higher concentration of substrate to achieve Vmax.If two enzymes in different pathways compete for the same substrate than values of Km and Vmax for both enzymes permit fate of the substrate.In given question all 3 GLUT1,GLUT1A,GLUT1B has same amino acid sequences despite mutation,as this is silent mutation no change in Km of enzymes would be there and all would most likely have same Km.But if it change Its structure than a mutant allele would produce a subunit that and if its own affinity was lower than it would inhibit the activity of its subunits at sub-saturating substrate concentration and act like a competitive inhibitor.

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