In the past lab or twoyou sent in DNA samples for sequencing. In the past when t

Question

In the past lab or twoyou sent in DNA samples for sequencing. In the past when the data came back a number of students had received a message from the sequencer that theirdata contained “multiple signals”. What is interesting about this is that it typically occurred on the T7 read and not the pKKrev read. As a case in point one read came back with a read that had multiple signals startingat 106 bp into the T7 read. The reverse read on the same sample, using pKKrev as the primer, did not have multiple signals at all. How can this data be explained?

Explanation / Answer

Answer:- This is the case of mixed DNA templates.

Causes of mixed DNA templates :

Solution for mixed template sequencing problems:

But in your case T7 is a forward primer that's why multiple signal starting into it. For a proper result you can do the following :

Hope this helps you in someway.

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