Date Section Lab 2 Review Name Different hand cleansing agents work in different

Question

Date Section Lab 2 Review Name Different hand cleansing agents work in different ways. Working as a group with the other students Different hand dle your bench, look up four of the agents you used in this lab, and determine what partof 6 ll was targeted by the agent. For example, did the agent destroy the cell membrane, or ribosomes and thus affect protein synthesis? Also find out whether the agent is bactericidal or bacteriostatic. Record this information in Table 2.2. cell was targ TABLE 2-2 Mechanism of Actio n of Hand Cleansers Bactericidal or bacteriostatic? Antibacterial effect on the cell Cleanser name Semal haum Hudvogen If bacteria still grew on your plate after you washed with an antibacterial soap, can you conclude that that organism is resistant to the soap tested? Explain your answer. How would you go about proving that organisms are resistant to a particular agent? 7 nortance of Hand Washing

Explanation / Answer

Ans-6

Bacteriostatic at low concentration or cidal at high concentration

Ans-7

Antibacterial soap consist of triclosan which having bacteriocidal effect, but on long standing use of antibacterial soap or another antibiotics may cause genetic mutation in bacteria which makes resistant to antibiotics by developing efflux pump in the cell membrane which prevent entry of antibiotic in bacteria cell.

Mechanism of action of hand cleanser Cleanser name Bacteriostatic or bacteriocidal antibacterial effect on cell Chlorhexidine At low concentration act as bacteriostatic(inhibit growth) while at Higher concentration act as bacteriocidal(kill bacteria) It releases positively charged molecules which bind with a negatively charged molecule in the cell wall Benzalkonium

Bacteriostatic at low concentration or cidal at high concentration

Disturb the intermolecular interactions which cause distort membrane bilayer structure so cellular component leak out, which result killing of bacteria Hydrogen peroxide bactericidal action is poor, it is bacteriostatic agent It is oxidative agent which destroy cell wall of bacteria 10% povidone Iodine Bacteriostatic at low conc, cidal at high concentration It destroys microbial protein and DNA

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