Q5. In eukaryotic organisms, there are three polymerases and each one binds to i
ID: 141448 • Letter: Q
Question
Q5. In eukaryotic organisms, there are three polymerases and each one binds to its own__________(one word), and produces a specific type of___________(one word) and the one that does mRNA transcription is (three words, please use the roman numberal format for the third "word")___ _______Q6. Which of the following does NOT occur in prophase of mitosis
A. Recombination B. mitotic spindle formation C. chromosomes condense D. All of the above happen in prophase of mitosis
Q7. Which of the following are required for DNA replication but NOT for Polymerase Chain Reaction?
dNTP (dATP, dGTP, dCTP, dTTP)
helicase
DNA polymerase I
SSBP
gyrase (topoisomerase)
primer (DNA or RNA)
DNA ligase
DNA polymerase III
Q5. In eukaryotic organisms, there are three polymerases and each one binds to its own__________(one word), and produces a specific type of___________(one word) and the one that does mRNA transcription is (three words, please use the roman numberal format for the third "word")___ _______
Q6. Which of the following does NOT occur in prophase of mitosis
A. Recombination B. mitotic spindle formation C. chromosomes condense D. All of the above happen in prophase of mitosis
Q7. Which of the following are required for DNA replication but NOT for Polymerase Chain Reaction?
dNTP (dATP, dGTP, dCTP, dTTP)
helicase
DNA polymerase I
SSBP
gyrase (topoisomerase)
primer (DNA or RNA)
DNA ligase
DNA polymerase III
Q5. In eukaryotic organisms, there are three polymerases and each one binds to its own__________(one word), and produces a specific type of___________(one word) and the one that does mRNA transcription is (three words, please use the roman numberal format for the third "word")___ _______
Q6. Which of the following does NOT occur in prophase of mitosis
A. Recombination B. mitotic spindle formation C. chromosomes condense D. All of the above happen in prophase of mitosis
Q7. Which of the following are required for DNA replication but NOT for Polymerase Chain Reaction?
dNTP (dATP, dGTP, dCTP, dTTP)
helicase
DNA polymerase I
SSBP
gyrase (topoisomerase)
primer (DNA or RNA)
DNA ligase
DNA polymerase III
Explanation / Answer
Answer 5) In eukaryotic organisms, there are three polymerases and each one binds to its own promoter, and produces a specific type of initiation complex and the one that does mRNA transcription is RNA Polymerase II.
Explanation for the answer:
In Eukaryotes there are three types of Ribonucleic acid (RNA) polymerases, namely, RNA polymerase I, II and III. They are mainly involved in the transcription process, in which an RNA template is synthesized from a deoxyribonucleic acid (DNA) strand. The transcription process starts when an RNA polymerase binds to specific sequences in a region known as the promoter, present on the DNA strand to be transcribed. After binding to the promoter, several accessory transcription factors also contribute for the formation of a pre-initiation transcription complex. Elongation and termination are the next two steps involved in the transcription process to result in the formation of an RNA transcript. Different RNA polymerases have different roles. RNA polymerase II helps in transcribing DNA to form a messenger RNA (mRNA) transcript.
Answer 6) Option (A) that is Recombination is the correct answer.
Explanation for the answer:
Mitosis is a process of nuclear division, exclusive to eukaryotic cells. This stage of the cell cycle is responsible for the duplication and production of two daughter cells from each parent cell. This process takes place in four consecutive steps, namely Prophase, Metaphase, Anaphase, and Telophase. Prophase is the first step of mitosis. In this step following events occur- nuclear envelope breakdown, condensation of chromosomes, spindle formation. The process of recombination occurs in meiosis. Hence, Option (A) that is Recombination is the correct answer.
Answer 7) Helicase, DNA polymerase I, Gyrase (topoisomerase), DNA ligase, DNA polymerase III are not used during Polymerase Chain Reaction (PCR).
Explanation for the answer:
PCR is a molecular technique used for exponentially amplifying a specific DNA segment into multiple copies (similar to the process of DNA replication in-vivo). Even though the principle of this technique is the same as that of DNA replication, it occurs differently by following three steps- Denaturation, Annealing, and Elongation. A different set of enzymes are also used as the PCR mixture. It includes DNA template, forward and reverse primers, buffers, Taq polymerase and deoxyribonucleotide triphosphate (dNTPs). Helicases and gyrases are not used for unwinding and rewinding of DNA strands as in PCR the strand separation is done by increasing temperature in the first step or denaturation. Since the DNA polymerases and ligases are enzymes, they fail to tolerate the high temperature in the denaturation step. Instead, a thermostable polymerase of Taq polymerase is used alternatively. Another reason for not using them is that in case of PCR, there are no Okazaki fragments (like in replication) to be ligated and rewound. Hence, Helicase, DNA polymerase I, Gyrase (topoisomerase), DNA ligase, DNA polymerase III are not used during Polymerase Chain Reaction (PCR).
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.