Biochemistry homework question!! Please help!!! The following table shows where

Question

Biochemistry homework question!! Please help!!!

The following table shows where different restriction endonucleases (restriction enzymes) cleave DNA. Th abbreviation R represents the purines (adenine and guanine). The pyrimidines (cytosine, thymine, and uracil) are abbreviated as Y. The abbreviation W represents adenine or thymine EnzymeTarget sequenceCleavage 5' GAATTC 3' 3' CTTAAG 5' EcoRI EcORV Haelll Hindlll PpuMI 5' G 3' CTTAA 5' GAT ATC 3' 3' CTA TAG 5' 5' GG CC 3' 'CC GG 5 5' A AATTC 3' G 5 5' GATATC 3' 3' CTATAG 5' 5' GGCC 3' 3. CCGG 5, 5' AAGCTT 3 3. TTCGAA 5' AGCTT 3' 3. TTCGA A 5 5' RGGWCCY 3 3. YCCWGGR 5 5' RG 3. YCCWG GWCCY 3' GR 5' Which restriction endonuclease(s) would cleave a DNA molecule at the following sequences? a) 5' GAAGATATCCTCGC 3' Haelll Hindll EcoRV EcoRI EcoRV Haell Hindill EcoRI continued below...

Explanation / Answer

a) 5' GAAGATATCCTCGC 3' -------- EcoRV cuts 5' GAAGAT ATCCTCGC 3'

b) 5' TCCAAGCTTGAATTC 3' ----------- HindIII cuts 5' TCCA AGCTTGAATTC 3'

EcoRI cuts 5' TCCAAGCTTG AATTC 3'

PpuMI recognizes the sequence

5' RGGWCCY 3'

3' YCCWGGR 5'

and it cuts 5' RG GWCCY 3'

3' YCCWG GR 5'

The sequence in sticky end from 5' to 3' is GWC where W is either adenine or thymine.

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