a)Some one has isolated proteins from a rapidly growing culture of cells and ana

Question

a)Some one has isolated proteins from a rapidly growing culture of cells and analyzed the N-linked oligosaccharide chains found on the glycosylated proteins. Not all of the chains are the same. Can you help out the researcher and hypothesize why they are different and where in the cells the proteins might have been at the moment the cells were homogenized and the proteins were isolated? Explain. b) How would you experimentally localize (visualize) within the golgi the mannosidase which takes off the first mannose residues of the core N-linked oligosaccharide chain – i.e. where and how would you find it in the golgi? When you do the localization experiment would you expect to find the mannosidase in a single golgi compartment? Explain. c) Do you expect to find the product of the mannosidase in the same place(s) that you find the enzyme? Explain?

Explanation / Answer

B. The structure of N-linked oligosaccharide is found to be same in animals and plants and in some single-celled eukaryotes, a branched oligosaccharide consist of 3 glucose, 9 mannose, and 2 N-acetylglucosamine. its biosynthesis begins in the cytosolic face of endoplasmic reticulum membrane. there will be the addition of one at a time at dolichol phosphate of 5 mannose residue and 2 N-acetylglucosamine.

and the further modification is done by Golgi complex of N-linked oligosaccharides

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