Which of the following statements is NOT correct regarding protein separation te
ID: 140595 • Letter: W
Question
Which of the following statements is NOT correct regarding protein separation techniques?
Two-dimensional gel electrophoresis is conceptually the same as combining gel filtration chromatography and ion exchange chromatography.
Western blotting is a useful technique to isolate proteins for use in downstream assays.
Specific antibodies are required for immunoblotting, antibody affinity chromatography as well as immunoprecipitation.
Gel filtration chromatography works on the same principle as polyacrylamide gel electrophoresis.
All the statements about enzymes are true EXCEPT:
a single enzyme typically reacts with many different substrates.
they lower the activation energy of a reaction.
they function in an aqueous environment.
they increase the rate of a reaction.
Which of the following statements about enzymes is FALSE?
The active site of an enzyme consists of the substrate binding site and the catalytic site.
An enzyme with two distinct substrates for which it has two Km values will have the same maximal reaction velocity.
The maximal velocity of an enzymatic reaction is dependent on the concentration of enzyme.
The Michaelis constant is an inherent property of an enzyme equal to the substrate concentration that yields maximal reaction rate.
Which of the following statements is NOT correct regarding protein separation techniques?
Two-dimensional gel electrophoresis is conceptually the same as combining gel filtration chromatography and ion exchange chromatography.
Western blotting is a useful technique to isolate proteins for use in downstream assays.
Specific antibodies are required for immunoblotting, antibody affinity chromatography as well as immunoprecipitation.
Gel filtration chromatography works on the same principle as polyacrylamide gel electrophoresis.
Explanation / Answer
Ans 1:
This statement is not correct :
Western blotting is a useful technique to isolate proteins for use in downstream assays.
Western blot is used to separate or isolate specifies protein from a given sample containing a mixture of proteins.
Other options are true.
Ans2:
All statements about enzymes are true EXCEPT:
A single enzyme typically reacts with many different substrates.
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