Which of the following statements is FALSE? a. A new RNA molecule can begin to b

Question

Which of the following statements is FALSE? a. A new RNA molecule can begin to be synthesized from a gene before the previous RNA molecule's synthesis is completed b. Nucleotide polymerization occurs only in the 3'-to-5' direction c. If two genes arc to be expressed in a cell, these two genes can he transcribed with different efficiencies. d. Unlike DNA, RNA uses a uracil base and a ribose sugar e. RNA polymerase is responsible for both unwinding the DNA helix and catalyzing the formation of the phosphodiester bonds between nucleotides The non-coding segments that are removed from mRNA transcript during maturation are: a) UTRs b) introns c) promoter d) exons e) all of the above Which of the following is required to make complementary DNA (cDNA) from RNA? a) restriction enzymes (endonucleases) b) reverse transcriptase c) DNA ligase d) gene cloning e) bacteria A laboratory might use dideoxyribonucleotides to _____. a) Separate DNA fragments b) Produce cDNA from mRNA c) Visualize DNA expression d) Sequence a DNA fragment e) Amplify DNA fragment In 1997, Dolly the sheep was cloned. Which of the following processes was used? a) Fusion of an adult cell's nucleus with an enucleated sheep egg. followed by incubation a surrogate b) Using induced pluripotent stem cells c) Replication and dedifferentiation of adult stem cells from sheep bone marrow d) Isolation of stem cells from a lamb embryo and production of a zygote equivalent e) None of the above

Explanation / Answer

1 answer

d) Unlike DNA, RNA uses a uracil base and a deoxyribose sugar.

RNA remains for ribonucleic corrosive. It is a critical atom with long chains of nucleotides.

A nucleotide contains

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2. answer

b. introns

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3. b. reverse transcriptase

A reverse transcriptase (RT) is a protein used to produce reciprocal DNA (cDNA) from a RNA layout, a procedure named switch interpretation. It is principally connected with retroviruses. Be that as it may, non-retroviruses additionally utilize RT (for instance, the hepatitis B infection, an individual from the Hepadnaviridae, which are dsDNA-RT infections, while retroviruses are ssRNA infections). RT inhibitors are generally utilized as antiretroviral medications. RT exercises are likewise connected with the replication of chromosome closures (telomerase) and some versatile hereditary components (retrotransposons).

Retroviral RT has three consecutive biochemical exercises:

(a) RNA-subordinate DNA polymerase action,

(b) ribonuclease H, and

(c) DNA-subordinate DNA polymerase action.

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4. answer : sequence DNA fragment

In the fundamental dideoxy sequencing response, an oligonucleotide preliminary is strengthened to a solitary stranded DNA layout and reached out by DNA polymerase within the sight of four deoxyribonucleoside triphosphates (dNTPs), one of which is 35S-marked. The response likewise contains one of four dideoxyribonucleoside triphosphates (ddNTPs), which end stretching when consolidated into the developing DNA chain. After fruition of the sequencing responses, the items are subjected to electrophoresis on a high-determination denaturing polyacrylamide gel and after that autoradiographed to imagine the DNA grouping. Three varieties of the dideoxy sequencing method are as of now being used and are displayed in this unit. In the "naming/end" system, groundwork chains are at first broadened and named without ending ddNTPs, while in the customary "Sanger" strategy, naming and end of preliminary chains happen in a solitary stride. A late variety of the dideoxy sequencing strategy is warm cycle sequencing in which the response blend, containing format DNA, preliminary, thermostable DNA polymerase, dNTPs, and ddNTPs, is subjected to rehashed rounds of denaturation, strengthening, and lengthening steps. The subsequent straight enhancement of the sequencing items permits significantly less format DNA to be utilized and takes out autonomous groundwork tempering and layout denaturation steps, which are required for the naming/end or Sanger strategies.

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5 answer

fusion of an adult cell's nucleus with an enucleated sheep egg, followed by incubation in a surrogate

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