218 etter i catin ns The table below summarizes the results of a replica-plating

Question

218 etter i catin ns The table below summarizes the results of a replica-plating experiment where a mixture of 347 E. col strains of different genotypes were tested for their ability to growth on a variety of media. Use this data to answer the following 5 questions (2.1 through 2 Mediunm Number of colonies that grew Complete medium (contains all amino acids, vitamins, and glucose as a carbon source) Minimal medium (glucose as a carbon source Minimal medium ampicillin (an antibiotic Minimal medium methionine (an amino acid Minimal medium +arginine (an amino acid Minimal medium +methionine +ampicillin Minimal medium arginine +ampicillin Question 2.1: How many colonies are auxotrophs? 347 261 51 303 301 57 64 A. 347 B. 261 C. 86 D. 51 E. 44 Question 2.2: How many colonies are wild type (met" arg Amp5)? A. 51 B. 210 C. 261 D. 296 E. 347 Question 2.3: How many colonies are met arg? A. 261 B. 303 C. 301 D. 42 E. 86

Explanation / Answer

2.1 Correct answer:- (B) 261
Explanation- An Auxotroph is an organism that have the ability to synthesize all the compounds needed for Growth.
-it is the opposite of prototroph.

2.2 Correct answer:- (A) 51
Explanation- wild type colonies are that which survive better in the presence of an antibiotic.

2.3 Correct answer:- (C) 301
Explanation:- met, arg colonies are 301.
-
, which is characterized by the ability to synthesize all the compounds needed for growth.

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