3. Blood cells (3 pts) Vertebrates (animals with skeletons) are all eukaryotes a
ID: 135994 • Letter: 3
Question
3. Blood cells (3 pts) Vertebrates (animals with skeletons) are all eukaryotes and have blood that contains red blood cells carrying hemoglobin. Here we will use non human blood but treat the material as a biohazard and follow all procedures as directed. View the prepared blood smear slide under 40X 100X, and 400X magnification. The cells wil appear pink to red and may be faint in color. A. Find and draw a few red blood cells (these are pinkish-red in color) B. Mature red blood cells do not contain a nucleus and are easily characterized by their toroid (doughnut) shape. What is the function of red blood cells and how is it possible that they function without a nucleus? C. Under 40X magnification find a white blood cell (nucleus is amorphous and stained purple). Draw and label the nucleus and note its unusual shape.Explanation / Answer
Below are your answers:
A) If the blood is from non human source then there are chances that you would find nucleus in them as not all organism will have non nucleated RBCs. If if taken from frog or any other amphibian/birds/reptile, RBCs will have nucleus but the shape that you'd find is mostly doughnut shape as it facilitates the diffusion of oxygen. Under the 400x microscope you woukd see the doughnut shaped numerous cell in pink color which will have dark red color stained nucleus( if the blood sample taken from an organism that has nuclear RBCs)
If the RBC is anucleated the the whole of the cell would appear red opaque doughnut structures.
Answer B)
Well, its true that the "mature" RBCs do not have nucleus as it requires more space inside cell to accomodate hemoglobulin protein which binds to oxygen, larger the space better is the volume of oxygen it can carry. As the main function of RBCs is to transport The hemoglobulin bound oxygen to all parts of body and flush out carbon dioxide from cells via expiration at lung. The proteins and enzyme that the cell requires is all made and stored during the period when the RBCs was still a precursor cell in bone marrow. So RBCs will have all important proteins and enzyme already stored and will use it only once in its life time of 120 days.
Answer c)
WBCs are cells that has many sub cells types, for e.g. nucleophils, esonophils, Basophils, monocytes, Lymphocytes etc. The most abundant of all is Nucleophil which is about 72-73% of the total WBCs and can easily be found in a blood smear which is stained. They have a large nucleus which is multilobulated and can be easily seen. Monocytes have large round shaped nucleus, Eosinophils will have red stained dumbbell shaped nuclei but these cells will be very less in numbers.
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