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1) The effectiveness of sanitation procedures in hospitals is determined by samp

ID: 135728 • Letter: 1

Question

1) The effectiveness of sanitation procedures in hospitals is determined by sampling surface for bacteria. Samples can be taken by rubbing a sterile, moistened cotton swab on a surface and inoculating a nutrient agar plate or with a Rodac environmental sampling plate. The 67-mm plates are designed so that an agar medium can be overfilled, producing a dome-shaped surface that ca be pressed onto a surface for sampling microbes. As the sanitation control officer, you test each method to determine which you will see. What can you conclude from these results?

Number of plates with colonies =5

Number of plates inoculated =50

Number of plates with colonies Number of plates inoculated swab onto nutrient agar 5 50 Rodac plate containig nutrient agar 6 40

Explanation / Answer

From these results, we can conclude that it will be more beneficial to use a Rodac plate as it allows for more growth of bacterial colonies in comparision to swab onto nutrient agar as Rodac plate is able to pick up more bacteria from the source directly without the need of using a swab.Also, there is less requirement of plates in Rodac in comparision to the swab plates.