Questions on SNPs I. Why have the genomics rescarchers chosen the namse, \"Singl
ID: 135629 • Letter: Q
Question
Questions on SNPs I. Why have the genomics rescarchers chosen the namse, "Single Nuxcleotide Polymorphisn,"to describe a genetic macleotide variation Questions for Vectors and Cloning rather ha, the word,-Mutation," as in penetics? I. What are the minimum charactcristics to make a fragment of DNA be useful as a 2. In the example of a phemotype for a SNP. ie. Imipramine, which patients, becomes toxic in a few paticnts, what causes the overdose of Imipramine? 2. When doing a bacterial transforimation by electroporation, what does the electri 3. Why should we try to transform E. coli with a recombinast plasmid when the bacteria are growing at mid-log phase? 3. Ia SNIP is located in an intron, is it likely to be clinically relevant? 4. You ligate YFG into a pTrelis vector,transform bacteria with it, and get lots of colonies. You added ampicillin, IPTG (artificial lactose), and X-Oal (another artificial results of your ligation? 4. If a SNP in the CYP2D6 gene results in a same sense codon (synonymous) in an eson, will this usaally result in a phenotype such as ADR (Adverse Drag Reaction)? 5. You cut a chromosome into pieces with the enzyme, EcoRl; and you cut your vector with Hind III. Then you ligate them together and run a gel to determine if there are approgriate ized bands (he size of vesclor plaus insert), The result is that you gt the original DNA fragments as before ligation. Why? How many people in this room do not have a SNP in any of their genes?Explanation / Answer
Answers in SNPs.
Answer 1)
Since each SNP is a difference in a single DNA building block, called a nucleotide.
For example, a SNP may replace the nucleotide cytosine (C) with the nucleotide thymine (T) in a certain part of DNA.
SNPs occur normally throughout a person’s DNA. They occur once in every 300 nucleotides on average that means there are roughly 10 million SNPs in the human genome. Most commonly, these variations are found in the DNA between genes. They act as biological markers, helping scientists locate genes that are associated with disease. When SNPs occur within a gene or in a regulatory region near a gene, they may play a more direct role in disease by affecting the gene’s function.
Most SNPs also don't show any effect on health or development. Some of these genetic differences, however, have proven to be very important in the study of human health. Researchers have found SNPs that may help predict an individual’s response to certain drugs, susceptibility to environmental factors such as toxins, and risk of developing particular diseases. SNPs can also be used to track the inheritance of disease genes within families. Future studies will work to identify SNPs associated with complex diseases such as heart disease, diabetes, and cancer.
Answer 2) eaths may occur from overdosage with this class of drugs. Multiple drug ingestion (including alcohol) is common in deliberate tricyclic overdose. As the management is complex and changing, it is recommended that the physician contact a poison control center for current information on treatment. Signs and symptoms of toxicity develop rapidly after tricyclic overdose. Therefore, hospital monitoring is required as soon as possible.
Answer 3) Yes, it will be relevant.
Firstly choose the important regions from the list of introns and confirm by Sanger Sequencing to exclude any NGS sequencing errors. You can also focus on coding SNPs, promoter SNPs, and miRNA binding regions and can decide how to prioritize them using bioinformatic tools.
Answer 4)yes it will show reaction towards ADR, by increasing transcription rate in downstream promoter.
Answer 5) 27-39% can suffer from SNPs.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.