Questions on Sanger Sequencing Questions on Flow Cytometry For Sangeer DNA seque
ID: 135625 • Letter: Q
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Questions on Sanger Sequencing Questions on Flow Cytometry For Sangeer DNA sequencing, what ingredients should be in the "G" tube? 1. When uning Buerescence, what physical property of a malecule ace you detecting and measuring 2. You have purified mRNA from cancer cels and converted the prep to cDNA Now you want to sequence them by Sanger sequencing method. Can you sequence the cDNA directly in this tube? 2. What part of a FACS machine excited the electrens in a melecule? 3. You are ready to sequence a cloned gene that no one has studied before. if no one 3. A phatemultiglier tube detects what physical element and cenverts it to an electrical knows the sequence how do you design a primer to start the reaction? signal? 4 Why do the dd-nucleotides terminate the symthesis of DNA during sequencing? 4. What does the FACS sorter do to get a desired cell into the correct collection tube? S. When reading a sequencing eel from radioactively labeled Sanger sequencing, where is s. On the dot plots, which is just a graph, what is graphed along the -axis or y asxin? the 5' end of the DNA sequence located on the gel?Explanation / Answer
Ans 1... ingredients of G tube
Primer and DNA template
DNA polymerase
ddNTPs with flourochromes
dNTPs for eg...d ATP,d CTP,dGTP,dTTP
Ans 2...No we cant sequence the cDNA directly in this tube.t he DNA sample is first divide into four separate sequencing reactions containing all 4 of the standards deoxynucleotide,(ddATP,ddGTP,ddCTP,ddzTTP)
Ans3...it very difficult to design a primer for unknown DNA sequence.in such cases u can use universal primer which works with most of the cases... examples of such sequence are..338F(ACTGTCACGGCGGCGGC)
Ans 4....The dd-nucleotides terminate the synthesis of DNA because it permanently bind to the active site of DNA polymerase
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