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1. All of the following may be termed Reducing Power Except? a. NADH b. FADH2 c.

ID: 13556 • Letter: 1

Question

1. All of the following may be termed Reducing Power Except?
a. NADH
b. FADH2
c. NADPH
d. CO2

2. After conversion of succinyl-CoA to succinate in step 5 of the TCA cycle, all of
the following have occurred in the cycle thus far (this step and the steps prior to
this step) EXCEPT?
a. ATP has been generated by substrate-level phosphorylation.
b. NADH has been generated.
c. CO2 has been generated.
d. A prosthetic group has been reduced.
e. an oxidative decarboxylation has occurred.

3-Which combination of reducing power can contribute the most to the proton
motive force? [*Assume that the system is an aerobically respiring yeast cell.]
a. The reducing power produced when two pyruvate molecules are converted
to 2 molecules of acetyl-CoA + the reducing power generated in one turn
of the TCA cycle.
b. The reducing power produced when one molecule of glucose is converted
to 2 molecules of pyruvate in the Embden-Meyerhoff pathway (glycolysis)
+ the reducing power produced when one pyruvate molecule is converted
to 1 molecule of acetyl-CoA.
c. The reducing power produced when one molecule of glucose is converted
to 2 molecules of pyruvate in the Embden-Meyerhoff pathway (glycolysis)
+ the reducing power generated in one turn of the TCA cycle.

4. As compared to substrate-level phosphorylation, oxidative phosphorylation
[*Please mark all that apply.]
a. generates ATP using the proton motive force as an energy source.
b. requires a molecule with a high phosphoryl group transfer potential to
donate a phosphoryl group to ADP.
c. occurs in step 7 of glycolysis.
d. Can only take place when there is an externally-derived electron acceptor
for the ETC.

5. Puspha is working in the lab with some proteins that she has isolated from the
mitochondria of Saccharoymces cerevisiae (yeast). Help her organize these
proteins in order of decreasing affinity for electrons
a. Cytochrome c, ubiquinone, NADH, cytochrome c oxidase
b. Cytochrome c oxidase, cytochrome c, ubiquinone, NADH
c. NADH, ubiquinone, cytochrome c, cytocrome c oxidase
d. Ubiquinone, NADH, cytochrome c, cytochrome c oxidase

6. A mutant strain of Saccharoymces cerevisiae (yeast) can be generated in which
the cytochrome bc1 complex retains its ability to transfer electrons but looses its
ability to pump protons. In such a mutant, which statements is/are TRUE?
a. This mutant would be incapable of reducing O2 and generating H2O.
b. In this mutant, the reduced form of cytochrome c would accumulate.
c. The complete oxidation of 1 NADH molecule by this mutant would result
in the pumping of 6 H+.
d. all of the above

Explanation / Answer

1. All of the following may be termed Reducing Power Except?
a. NADH
b. FADH2
c. NADPH
d. CO2

2. After conversion of succinyl-CoA to succinate in step 5 of the TCA cycle, all of
the following have occurred in the cycle thus far (this step and the steps prior to
this step) EXCEPT?
a. ATP has been generated by substrate-level phosphorylation.
b. NADH has been generated.
c. CO2 has been generated.
d. A prosthetic group has been reduced.
e. an oxidative decarboxylation has occurred.

3-Which combination of reducing power can contribute the most to the proton
motive force? [*Assume that the system is an aerobically respiring yeast cell.]
a. The reducing power produced when two pyruvate molecules are converted
to 2 molecules of acetyl-CoA + the reducing power generated in one turn
of the TCA cycle.
b. The reducing power produced when one molecule of glucose is converted
to 2 molecules of pyruvate in the Embden-Meyerhoff pathway (glycolysis)
+ the reducing power produced when one pyruvate molecule is converted
to 1 molecule of acetyl-CoA.
c. The reducing power produced when one molecule of glucose is converted
to 2 molecules of pyruvate in the Embden-Meyerhoff pathway (glycolysis)
+ the reducing power generated in one turn of the TCA cycle.

4. As compared to substrate-level phosphorylation, oxidative phosphorylation
[*Please mark all that apply.]
a. generates ATP using the proton motive force as an energy source.
b. requires a molecule with a high phosphoryl group transfer potential to
donate a phosphoryl group to ADP.
c. occurs in step 7 of glycolysis.
d. Can only take place when there is an externally-derived electron acceptor
for the ETC.

5. Puspha is working in the lab with some proteins that she has isolated from the
mitochondria of Saccharoymces cerevisiae (yeast). Help her organize these
proteins in order of decreasing affinity for electrons
a. Cytochrome c, ubiquinone, NADH, cytochrome c oxidase
b. Cytochrome c oxidase, cytochrome c, ubiquinone, NADH
c. NADH, ubiquinone, cytochrome c, cytocrome c oxidase
d. Ubiquinone, NADH, cytochrome c, cytochrome c oxidase

6. A mutant strain of Saccharoymces cerevisiae (yeast) can be generated in which
the cytochrome bc1 complex retains its ability to transfer electrons but looses its
ability to pump protons. In such a mutant, which statements is/are TRUE?
a. This mutant would be incapable of reducing O2 and generating H2O.
b. In this mutant, the reduced form of cytochrome c would accumulate.
c. The complete oxidation of 1 NADH molecule by this mutant would result
in the pumping of 6 H+.
d. all of the above