Below is a map of a plasmid vector and a gene-of-interest that you want to inser

Question

Below is a map of a plasmid vector and a gene-of-interest that you want to insert into the plasmid. Numbers indicate the length in base pairs of specific areas of each map. EcoRI, Pvull, and Clal are restriction enzyme sites. Use this information to answer the following questions. 6. EcoRI EcoR EcoRI Clal 300 600 Pvull 200 900 bp 1200 bp 1000 How many DNA fragments are formed and what is the size of each fragment in base pairs (bp) when plasmid alone is treated with EcoRI? Parts a-b pertain to the plasmid alone. a. be 1200bp How many DNA fragments are formed and what is the size of each fragment in base pairs (bp) when plasmid alone is treated with Pvull? b. nOU Ore ut ore Gragrent Se ha be 12o0 bp How many DNA fragments are formed and what is the size of each fragment in base pairs (bp) when the insert is treated with EcoRI? Parts c-d pertain to the DNA insert alone. c.

Explanation / Answer

a) When the plasmid is cut with EcoR1, one DNA fragment will be formed 1200 bp length.

b) When the plasmid is cut with PvuII, one DNA fragment Will be formed with 1200 bp length.

C) When the insert is cut with EcoR1, one DNA fragment Will be obtained with 900 bp length.

D) when the insert is cut with ClaI, two dna fragments will be obtained. One will be 300 bp and other will be 600 bp in length.

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