Biochemistry Question (Citric Acid Cycle/Krebs Cycle) 1. Which step of the citri
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Question
Biochemistry Question (Citric Acid Cycle/Krebs Cycle) 1. Which step of the citric acid cycle has SUBSTRATE LEVEL PHOSPHORYLATION? 2. How many cycles of the citric acid cycle are required to eliminate 2 carbons contained in the acetate as CO2?Biochemistry Question (Citric Acid Cycle/Krebs Cycle) 1. Which step of the citric acid cycle has SUBSTRATE LEVEL PHOSPHORYLATION? 2. How many cycles of the citric acid cycle are required to eliminate 2 carbons contained in the acetate as CO2?
1. Which step of the citric acid cycle has SUBSTRATE LEVEL PHOSPHORYLATION? 2. How many cycles of the citric acid cycle are required to eliminate 2 carbons contained in the acetate as CO2?
Explanation / Answer
1. Krebs cycle is also known as Citric Acid Cycle or Tricarboxylic Acid Cycle. It is a step wise cyclic process which is used to oxidize the pyruvate. Therefore, it is also known as citric acid cycle. Krebs cycle is a stepwise cyclic oxidation process in which nine steps occur in overall Krebs cycle:
In the sixth step of the citric acid cycle has SUBSTRATE LEVEL PHOSPHORYLATION.
n the presence of enzyme succinyl thiokinase, succinyl CoA is hydrolyzed. CoA and Succinate are formed. The energy liberated during the process is used in synthesis of ATP in Plants and GTP (Guanosine triphosphate) or ITP (Inosine triphosphate) in animals and CoA is also released out.
2. Each turn of the citric acid cycle produces three NADH and one FADH2, as well as one GTP (or ATP). Two CO2 are released in oxidative decarboxylation reactions. Therefore, only one cycles of the citric acid cycle are required to eliminate 2 carbons contained in the acetate as CO2.
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