Part II 3 1. Sanger sequencing: The denaturing gel at right shows the products o

Question

Part II 3 1. Sanger sequencing: The denaturing gel at right shows the products of four polymerization termination sequencing reactions (Sanger technique). Lane 1 holds the products of Reaction 1, etc. In each case, DNA polymerase was used to create fragments of random length from template DNA with an unknown sequence. All four dNTPs were provided in all reactions (dATP, dCTP, dGTP and dTTP). Each reaction also contained a small amount of ONE Reaction 1: ddCTP Reaction 2: ddTTP Reaction 3: ddGTP Reaction 4: ddATP a.What is the (5' ->3') sequence of the template ssDNA? Show and explain your work. b. this problem, and explain how they would change the data or readout. List at least 2 real-life complications that we're ignoring in

Explanation / Answer

the first lane corresponds to C, 2nd to T, 3rd to G and 4th to A, the sequence would be started to be considered from the bottom. The sequence is as follows:

A G T G A T C G C C T G T C A C G A G A T C C G T A G G C A G C

2. Real life problems in this sequencing is:

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