6:23 Lab 3- Aseptic Technique Post Lab Report (5pts) List 3 three reasons why th

Question

6:23 Lab 3- Aseptic Technique Post Lab Report (5pts) List 3 three reasons why the use of aseptic technique is essential when handling microbial cultures in the laboratory 2: 3: 2 (a) Define the word inoculate (b) use the word inoculate in a sentence that relates to a Which tool would you use to transfer bacteria from one tube of broth to another, a loop or a needle? Why? Which tool would you use to transfer bacteria from an agar plate to a tube of broth, a loop or a needle? Why? s If you are holding the inoculating loop in your right hand, which finger and which hand should be used to remove the cap from the tube you are inoculating and holding in your left hand Name: When you are transferring microbes f should you not set the cap on the Section: AM PM Cirele the correct answer regarding the sterilization of an inoculating loop: The loop itself should be flamed for 1-2 seconds Both the loop and the wire should be flamed until they are red hot o The wire, but not the loop, should be flamed for 1-2 seconds o It is not necessary to ever sterilize the inoculating loop Consulting the results in your lab notebook from this exercise, did you find contamination anywhere? think caused the contamination. Ifso, describe what you

Explanation / Answer

Ans.1

Aseptic technique is essential as it insures that:

1. No contaminating organisms are introduced into the culture.

2. It Does not contaminate the person handling and

3. No contamination remains after.

Ans.2.(a)

To innoculate means to introduce antigen or serum or vaccine into a person or animal to provide immunity against a specific disease.

Inoculate also means to introduce microorganisms into a growth medium.

Ans.2.(b).

Inoculate the microorganisms into the laboratory animals.

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