5-2. Researchers have recently published that glioma cells (brain cancer) displa

Question

5-2. Researchers have recently published that glioma cells (brain cancer) display an upregulation of CapG protein. CapG is an actin capping protein that resembles gelsolin. CapG is overexpressed (more than normal protein levels) in glioma cells. a. The figure below shows how different amounts of CapG in a cell can influence cell migration. Cells are grown until they evenly cover the bottom of a petri dish. Then, cells are scraped off and allowed to fill in the hole which simulates wound healing. Pictures were taken after 4 hours (first row) and 24 hours (second row). Then researchers can monitor how many cells fills in the hole after 24 hours (second row below). Anlyze the figure below. Compare cell migration (how well the cells filled in the gap) between cells depleted of CapG and cells that overexpress CapG. Rubric (2): Correct comparison of the amount of migration observed in CapG depleted cells when compared with cells overexpressing CapG Depleted CapG (less) Depleted CapG (less) Overexpressed CapG (more) Control 4 htze 24 h

Explanation / Answer

A)Th action protein binding capG modulates cell migration and movement by interacting with the cytoskeleton.overexpression of capG markedly promoted cell growth in vitro and depletion of capG significantly inhibted the proliferation by blocking the cell cycle at G1/S transition

B)actin capping and actin severing could increase cell migration as the motile cells give a leading edge and produce a vranched network of actin filaments that gives rise to physical force as the polymer continues to grow beneath the plasma membrane.

C)lamellopodium and fillopdium are seen on the edges and stress fibres in the middle.so most actin filaments are seen at the edges.

D)motility is initiated by an actin-dependent protrusion of the cell’s leading edge, which is composed of armlike structures called lamellipodia and filopodia. These protrusive structures contain actin filaments, with elongating barbed ends orientated toward the plasma membrane.During cellular arm extension, the plasma membrane sticks to the surface at the leading edge.nucleus and the cell body are pushed forward through intracellular contraction forces mediated by stress fibers.Then, retraction fibers pull the rear of the cell forward.

The nucleotide state of actin would include ATP and ADP(I)

E)answered in above options

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