iz Instructions Question 3 1 pts PCR is used for cloning DNA and after about 30
ID: 132886 • Letter: I
Question
iz Instructions Question 3 1 pts PCR is used for cloning DNA and after about 30 cycles, the number of copies of DNA will have increased about a billion times. Why is the DNA polymerase from the bacteria Thermus aquaticus used in PCR? O Due to the walls of the he plant cell, which are thick; therefore it is used to degrade the walls for PCR to begin. O It is present and needed because the enzyme is resistant to very high temperatures since the bacteria live in hot springs. O It allows scientists to be able to determine the variable number of tandem repeats of minisatellites or microsatellites to determine an individual's DNA fingerprint. It is used to flank the gene to be copied. Because it uses primers specific to sequences in a disease causing gene or organism to diagnosis diseases, etc. 5Explanation / Answer
Answer: Option B is correct.
Explanation:
PCR is used to amplify target gene sequences. It is an invaluable technique used in molecular biology and genetics.
PCR reaction components:
Taq polymerase
Buffer
iii. dNTPs
iv. Primers
v. Template
PCR steps:
i. Denaturation (92'C to 94'C): Unwinding of the DNA duplex
ii. Annealing (50'C to 65'C): Binding of the primer to the template DNA
iii. Extension (70'C to 72'C): Extension of the primer by Taq polymerase
iv. Repeat
Primer is a short oligonucleotide sequence (15 to 30 ntd) that binds specifically to the template DNA using sequence complementarity. The primer binds to the template DNA only when the temperature is below the Tm of the DNA-primer duplex.
The Taq polymerase is a thermostable DNA polymerase isolated from Thermus aquaticus. The enzyme shows optimum activity at 72’C which is required at the elongation step of the PCR reaction. Normal DNA polymerases isolated from mesophilic organisms would generally be denatured at this temperature. Hence Taq polymerase is used.
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