i) Aseptic Technique i) Axenic Culture ii) Complex or Nutrient media v) Anaerobi
ID: 132644 • Letter: I
Question
i) Aseptic Technique i) Axenic Culture ii) Complex or Nutrient media v) Anaerobic v) Defined/Synthetic media Q3. What is the purpose of oil immersion with the microscope that you use in your class? Whyt can't you view viruses with your microscope? Q4. What is the totai magnification of your microscope if the ocular has 10X, the objective lens is 43X and the condenser lens is 20X. Q5. Why is it required to fix the bacterial smear on the slide, What is the advaatage or disadvantage to "Heat fix" or "Air dry" bacterial smears. What may happen during staining if the smear is not fixed?Explanation / Answer
According to the Chegg guidelines, if multiple questions are posted, it is appropriate to answer first full question and hence i will be answering Q3 completely.
Oil immersion is a method to increase the microscope resolving power. This is done by immersing both the objective lens and the specimen in a transparent oil which is having high refractive index which in turn increases the objective lens numerical aperture.
Immersing oil is transparent and has specific optical and viscosity features which is necessary for the use in microscope. It will have an refractive index around 1.515.
It is true that viruses can't be viewed in the light microscope that is used in the class. It requires electron microscope. Viruses have a rough size of 1/100 of bacteria, so they are too small to be viewed under light microscope. According to the information provided by wikipedia the utmost limit with light microscope is approximately 1500x magnification. Most of the viruses are smaller like influenza A is of size around 80-120nm, HIV viruses is of 120nm, the rhinovirus is around 30nm. They can be viewed only using electron microscope.
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