C. Addition or deletion of a sin le nucleotide or two nucleotides change the rea

Question

C. Addition or deletion of a sin le nucleotide or two nucleotides change the reading frame to aubhs could affect the same amino acid. make a non-functional protein. D. Addition or deletion of three nucleotides do not change the reading frame. E. Both C and D 25. The strand shown is the RNA-lik stop codons) exist that extend through the following sequence? e strand. How many possible open reading frames (frames without A. 0 B. 1 C. 2 D. 3 26. What information cannot be determined or predicted from the DNA sequence of a cDNA clone? A. exon sequences B. sequence of the promoter C. intron sequences D. amino acid sequence of the encoded polypeptide E. Both B and C 27. What is the average size of fragments if a random sequence of DNA is digested with a restriction enzyme that has a 4-base recognition site? A. 6 bp B. 24 bp C. 256 bp D. 4096 bp 28. What is the approximate number of fragments made if 1kb DNA is digested with a restriction enzyme that has a 4-base recognition site? A. 1 fragment B. 2 fragments C. 4 fragments D. 6 fragments E. 8 fragments

Explanation / Answer

Que 25

Ans:

The strand shown is the RNA-like strand. Possible open reading frames (frames without stop codons) exist that extend through the following sequence is: A. 0.

Explanation:

- The region of the nucleotide sequences from the start codon to the stop codon is called Open reading frame (ORF).

- Thus, an ORF is a sequence of DNA that starts with start codon.

- Hence, correct option is: A.

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