7. Gene transcription starts at a and ends at a a. start codon, terminator d. en

Question

7. Gene transcription starts at a and ends at a a. start codon, terminator d. enhancer, silencer b. start codon, stop codon e. promoter, terminator c. promoter, stop codon 8. In prokaryotes, the function of the Shine-Delgarno sequence is to: a. enhance RNA stability d. trigger mRNA degradation b. allow DNA unwinding e. promote ribosome binding c. recognize sigma factor 9. The function of the consensus sequence (AAUAAA) located near the 3' end of a pre-mRNA is to promote which event(s)? a. RNA cleavage d. a and b only b. addition of the Poly-A tail e. a, b, and c c. transcriptional termination 10. The requirements for normal pre-mRNA splicing are a. 5' splice site in the intron c. branch point adenosine in the intron b. 3' splice site in the intron d. spliceosome complex in the nucleus e. all of these choices 11. Which RNA processing event(s) use a guide RNA to modify sequences after transcription? c. RNA editing a. alternative splicing d. RNA interference b. RNA methylation e. adding the Poly-A tail Page 1 of

Explanation / Answer

7) The answer is e.The transcription starts at promoter where RNA polymerase binds and transcribe the gene and ends ar terminator.START and STOP codon play an important role in translation.

8) The answer is e. It is the binding site for Ribosome.It is upstream to START Codon (AUG).

9) The answer is e.Addition of poly A tail is called polyadenylation which is essential for producing mature m-RNA.For polyadenylation it is essential that the mRNA is to be cleaved by some proteins.

10) The answer is e. Splicing is the removal or excision of introns an ligation of exons to form mature mRNA so that it can be translated into protein.It requires all of the above choices.The 5' end of intron include a invariant sequence GU and 3 ' splice site include AG sequence and there is a pyrimidine rich region called pyrimidine tract upstream to AG sequence at 3' splice site.And upstream to pyrimidine tract is branch point.

11) The answer is c (RNA editing). It includes the editing of RNA molecules through insertion,deletion,base substitution but it does not include splicing , polyadenylation.

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