05. A schematic of a DNA binding protein is shown in the figure. X residues in t

Question

05. A schematic of a DNA binding protein is shown in the figure. X residues in this protein are depicted as dark circles. I make a mutant form of the protein in which all the Xs shown are changed to Ys. 06. This enzymatic reaction (A+BDC) has reached equilibrium. The ?G° of the reaction is 20 kJ/mol, R = 8.314 k]/K/mol, T- 298 K. 07. The same protein sample was run on two different Gel type 1 Reduced condition Gel type 1: Gel type 2: gels types. With Gel type 1, the samples were also run under both reduced and non-reduced conditions. Da L 100 75 |-- 50 25 1001- 75 100 75 50 25 25

Explanation / Answer

The explanation given in the above diagram that your sample has two protein.  The one with desired size will be the protein of your interest while the other could be a contaminant. This can be separated by the size exclusion chromatography as well as by anion exchange chromatography as they are totally different from each other.

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