Question 3 is what I’m looking for. Why would one dye elute before another? And

Question

Question 3 is what I’m looking for. Why would one dye elute before another? And what signs during the experiment would point me towards this? Ion-Exchange Chromatography You had 500 L of red/blue dye Results. The mg blue dye, % recovery, relative pl of red and blue dye (greater than xx or less than xx); also which dye eluted first. Discussion: Why XX dye eluted first, why didn't the YY dye elute, explain theory of yy dye removal and relationshio to pl. Spreadsheet of all raw data and calculation of percent recovery and amount of blue dye Data Presentation: Standard Curve 1. 2. 3. 4. 5.

Explanation / Answer

The reason that one dye elutes before another is that the polarities of both the dyes are different. The two dyes bind to the column and as the polarity of the buffer is decreased, the dyes elute from the column. The dyes have structural differences and generally, the red dye is more polar than the blue dye. Consequently, the red dye detaches from the substrate and elutes from the column when the polarity of the surrounding liquid decreases. When the polarity of the solution further decreases, the blue dye elutes from the column.

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