List the amino acids with the 3 largest side chains Identify the one with the la

Question

List the amino acids with the 3 largest side chains Identify the one with the largest Rf value and compare the polarity of its side chain to the other two. Then list the amino acids with the three most polar side chains Identify the one with the largest Rf value and compare its size to the other two amino acids. If information is not clear can you please tell me exactly what you need to know?

abie 1BODtS.total Size ranking Polarity (P, NP) de (cm) d (cm) Amino Acid (1-8) unne 8.90 1.00 0.809 Aseactu 3 P 3.30 130 0,35 Seriae Lysine Unknown spot 1 Unknown spot 2 5.50 11. 0D 0 10.91 0.32l0 1.80 10.1 0,210 1. State the unknown code, identify the unknowns and justify reasons for arriving at your code i>urknoon #1 conclusion. (4 pts.) Unknown un known s t S

Explanation / Answer

Tf values

Given above is the table with Rf values for some of the amino acids

As can be seen as the hydrocarbon side chain of the amino acid increases, it becomes less polar and thus moves faster on the plate, giving high Rf values.

As the polarity of the side chain increases, the amino acid interacts more strongly with the stationary phase on the plate and thus moves slower, giving low Rf values.

The amino acids with large side chains tryptophan, tyrosine and leucine. Tryptophan has the highest Rf value of all. It is very close to the Unknown spot 1 Rf value and most likely the identity of the unknown spot 1 is tryptophan.

Unknown spot 2 has the Rf more identical to aspartic. Therefore the side chain of aspartic and the unknown spot 2 might be similar.

Overall as the size of the side chain of the amino acid increases, the Rf value was also found to incease due to decreasing polarity of the amino acid.

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