2. (4 pts), Olaf is going to be running an enzyme assay that will have a final v

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2. (4 pts), Olaf is going to be running an enzyme assay that will have a final volume of 300uL. This assay will require the substrate o-nitrophenyl-ß-galactoside, and luckily there is a stock solution of this already prepared which has a concentration of 2.65 mM. Olaf has been instructed to add 50 uL of this substrate stock substrate to each of his assay reactions. Help Olaf determine the number of nmoles of o-nitrophenyl-ß-galactoside that will be in each of the assay reactions. 3. Serial dilutions are often used in a laboratory as they provide a fairly accurate way to prepare very dilute samples. In a ten-fold serial dilution a stock solution of 1M would be sequentially diluted 10-fold to 0.1 M, then this solution would be dilute 10-fold to 0.01M, which then could be diluted to 0.001M, and so on. a). (2 pts) Assist Elsa perform a five-fold serial dilution of a protein solution that has a starting concentration of 2 mg/mL. Elsa plans on making four (serial) dilutions of her stock, calculate the concentrations of each these dilutions for Elsa. Concentration (mg/mL) 2 mg/mL Dilution Number Stock solution Dilution 1 Dilution 2 Dilution3 Dilution 4 b).(3 pts) Show Elsa exactly how to make up 75 uL of dilution 4. Use milli-Q for diluting.

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2. (4 pts), Olaf is going to be running an enzyme assay that will have a final v

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